NAVIGATIOn

| CRYSTALLOGRAPHY | NMR | BIOPHYSICS | CHEMICAL SYNTHESIS |

| BIOINFORMATICS | ENZYME SCREENING |

 

Structural biology

 

The macromolecular crystallography facility

The facility possesses all the necessary equipment to overcome the different steps leading to the crystal structure of any purified target. This includes:

  • Nano-dispensing crystallization robots: Innovadyne Nanodrop ExtY and TTPLabtche Mosquitoe (equipped for LCP).
  • System for visualization and management of crystallization trials: TriTek CrystalPro XS.
  • Fully automated storage and imaging system: Formulatrix Rock Imager 1000 equipped with visible and UV Optics.
  • Thermoregulated rooms at 4, 12 and 20 °C for the setting up of automated and manual crystallization trials.
  • In-house X-ray data collection and processing equipment: a 4-circle diffractometer with CCD detector (XCalibur Nova, Oxford Diffraction/Agilent), a diffractometer specifically designed for the identification and analysis of protein crystals within multi-well crystallization plates (PX Scanner, Oxford Diffraction/Agilent).
  • Binocular microscopes, cryogenic containers for storage and shipping of crystals.

The facility has access through individual or Block Allocation Group (BAG) proposals to several French and European synchrotrons (ESRF, Grenoble; SOLEIL, Gif/Yvette; ALBA, Barcelona; DESY, Hamburg).

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The macromolecular crystallography facility

The facility possesses all the necessary equipment to overcome the different steps leading to the crystal structure of any purified target. This includes:

The facility has access through individual or Block Allocation Group (BAG) proposals to several French and European synchrotrons (ESRF, Grenoble; SOLEIL, Gif/Yvette; ALBA, Barcelona; DESY, Hamburg).

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The NMR facility

The facility contains three high-field state-of-the-art NMR spectrometers for data acquisition and computers for NMR analyses. The instrumentation presently available includes four-channel Bruker Avance 500, 600, and 700 MHz NMR spectrometers, devoted to high-resolution liquid-state and solid-state NMR. This equipment associated with various probe heads offers a large range of capabilities for biochemical analyses and structural biology.

The 500 and 700 MHz are versatile spectrometers that can be used for both liquid and solid samples (heterogeneous samples, amyloid fibrils, tissues, in-cell NMR, etc) depending on the probe installed. For that purpose, they are equipped with static and MAS probes with various spinning rates, from 12 KHz (4 mm O.D. rotor) to 60 KHz (1.3mm O.D. rotor) with two channels (X: broadband) to three channels (1H, 13C, 15N).

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biophysical facility

The equipment of the biophysical facility includes variety of instruments, which allow to probe the homogeneity and thermostability of the samples and to screen or characterize ligand-protein interactions using complementary approaches.

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chemical facility

Automated chemical synthesys

Chemical library

Chromatography equipments

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chemo-informatics facility

Equipment of the chemo-informatics facility includes two clusters for computational tasks as well as GPU servers.

Clusters

Softwares

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The enzyme screening facility

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